| American Association for Dental Oral and Craniofacial ResearchAlexandria | Form 990 | $2,622,891FY2024 |
| Center for Advancing Public HealthFairfax | Form 990 | $2,488,969FY2024 |
| Richmond and Henrico Public Health FoundationRichmond | Form 990 | $2,420,863FY2025 |
| National Association of State Head Injury AdministratorsAldie | Form 990 | $2,405,524FY2024 |
| Childrens Health Investment Program IncChesapeake | Form 990 | $2,297,482FY2025 |
| Tidewater Emergency Medical Services Council IncChesapeake | Form 990 | $2,289,170FY2025 |
| Northern Virginia Dental Clinic IncFairfax | Form 990 | $2,276,657FY2025 |
| Piedmont Regional Dental Clinic IncOrange | Form 990 | $2,225,778FY2025 |
| Bay Rivers Telehealth AllianceTappahannock | Form 990 | $2,181,211FY2025 |
| John Randolph Foundation IncHopewell | Form 990 | $2,180,662FY2025 |
| Augusta Regional Free Clinic IncFishersville | Form 990 | $2,176,502FY2025 |
| Free Clinic of PowhatanPowhatan | Form 990 | $2,080,697FY2025 |
| Cloverleaf Equine CenterClifton | Form 990 | $2,060,392FY2024 |
| Western Virginia Emergency Medical Services Council IncRoanoke | Form 990 | $2,008,154FY2025 |
| American Trauma SocietyFalls Church | Form 990 | $1,945,514FY2025 |
| Community Futures FoundationRichmond | Form 990 | $1,907,792FY2025 |
| Sprout Therapeutic Riding and Education CenterAldie | Form 990 | $1,832,551FY2024 |
| Bland County Volunteer Rescue Squad IncBland | Form 990 | $1,821,058FY2025 |
| Edmarc IncPortsmouth | Form 990 | $1,777,212FY2025 |
| Mel Leaman Free Clinic of Smyth County IncMarion | Form 990 | $1,717,151FY2025 |
| Assist Crisis Pregnancy CenterAnnandale | Form 990 | $1,715,694FY2025 |
| Virginia Center for Health InnovationMidlothian | Form 990 | $1,714,759FY2025 |
| Harrisonburg Rescue Squad IncorporatedHarrisonburg | Form 990 | $1,699,129FY2024 |
| Waynesboro First Aid Crew IncWaynesboro | Form 990 | $1,688,625FY2024 |
| Care Net Resource Pregnancy Centers IncNewport News | Form 990 | $1,651,399FY2025 |
| Fredericksburg Christian Health CenterFredericksburg | Form 990 | $1,643,163FY2024 |
| Friends of Guest House IncAlexandria | Form 990 | $1,614,024FY2025 |
| American Art Therapy Association IncArlington | Form 990 | $1,550,830FY2025 |
| Southside Rescue Squad IncSouth Hill | Form 990 | $1,548,246FY2025 |
| Virginia Beach Rescue Squad IncVirginia Beach | Form 990 | $1,478,127FY2024 |
| Virginia Hospital Center FoundationArlington | Form 990 | $1,475,826FY2024 |
| Mother Health International IncorporatedVienna | Form 990 | $1,464,842FY2025 |
| Fairview Home IncBlacksburg | Form 990 | $1,461,545FY2025 |
| Commonshare IncHenrico | Form 990 | $1,461,443FY2024 |
| Bristol Life Saving Crew IncBristol | Form 990 | $1,456,232FY2025 |
| Pro Choice Virginia FoundationAlexandria | Form 990 | $1,448,732FY2024 |
| Crisis Pregnancy Center of Prince William CountyManassas | Form 990 | $1,423,731FY2024 |
| Adams Compassionate Healthcare NetworkChantilly | Form 990 | $1,366,424FY2025 |
| North American Transplant Coordinators OrganizationOak Hill | Form 990 | $1,323,017FY2024 |
| Moore House IncPortsmouth | Form 990 | $1,322,861FY2025 |
| Old Dominion Emergency Medical Services Alliance IncHenrico | Form 990 | $1,318,331FY2025 |
| Healthy Families Rappahannock AreaFredericksburg | Form 990 | $1,317,211FY2025 |
| Organization for Spinal Hygiene Awareness IncStafford | Form 990 | $1,314,374FY2025 |
| Virginia Telehealth NetworkNorth Chesterfield | Form 990 | $1,290,265FY2024 |
| Pregnancy Resource Center of Metro RichmondHenrico | Form 990 | $1,268,583FY2024 |
| Middlesex County Volunteer Rescue Squad IncDeltaville | Form 990 | $1,259,509FY2025 |
| Blue Ridge Womens Center IncRoanoke | Form 990 | $1,250,148FY2024 |
| Ronald McDonald House Charities of Norfolk Virginia IncNorfolk | Form 990 | $1,240,125FY2025 |
| Equi-Kids Therapeutic Riding ProgramVirginia Beach | Form 990 | $1,215,403FY2025 |
| Birth in Color Rva FoundationRichmond | Form 990 | $1,196,187FY2024 |