| National Wildlife FederationReston | Form 990 | $124,061,599FY2025 |
| People for the Ethical Treatment of Animals IncNorfolk | Form 990 | $110,179,122FY2025 |
| Trout Unlimited IncArlington | Form 990 | $109,528,553FY2025 |
| American Bird ConservancyMarshall | Form 990 | $26,955,379FY2024 |
| Sea Shepherd Conservation SocietyWoodbridge | Form 990 | $15,110,537FY2024 |
| Richmond Society for the Prevention of Cruelty to AnimalsRichmond | Form 990 | $14,304,512FY2025 |
| Anicira Veterinary CenterHarrisonburg | Form 990 | $12,110,566FY2025 |
| Colbys Crew RescueKeswick | Form 990 | $11,350,208FY2024 |
| Paws of Honor IncWilliamsburg | Form 990 | $10,592,256FY2024 |
| Gloucester-Mathews Humane SocietyGloucester | Form 990 | $7,593,689FY2025 |
| Virginia Beach SPCAVirginia Beach | Form 990 | $7,169,374FY2025 |
| Albemarle Society for the Prevention of Cruelty to AnimalsCharlottesville | Form 990 | $7,047,808FY2025 |
| Cheetah Conservation FundAlexandria | Form 990 | $6,714,660FY2024 |
| Lynchburg Humane Society IncLynchburg | Form 990 | $6,698,300FY2025 |
| Bristol Humane SocietyBristol | Form 990 | $5,884,818FY2025 |
| Animal Welfare League of Arlington VA IncorporatedArlington | Form 990 | $5,564,401FY2025 |
| Virginia Zoological SocietyNorfolk | Form 990 | $5,335,550FY2025 |
| Humane Farm Animal CareMiddleburg | Form 990 | $5,335,113FY2025 |
| Lucky Dog Animal RescueArlington | Form 990 | $5,189,099FY2025 |
| Richmond Animal League IncNorth Chesterfield | Form 990 | $5,011,368FY2025 |
| Animal Welfare League of Alexandria Virginia IncorporatedAlexandria | Form 990 | $4,728,224FY2025 |
| Assisi Animal Clinics of Virginia IncRoanoke | Form 990 | $4,698,668FY2025 |
| Wildlife Center of VirginiaWaynesboro | Form 990 | $4,434,174FY2024 |
| Middleburg Humane FoundationMarshall | Form 990 | $4,000,353FY2024 |
| Mutts with a MissionVirginia Beach | Form 990 | $3,622,106FY2024 |
| Anicira Veterinary Center - California IncHarrisonburg | Form 990 | $3,394,063FY2025 |
| Rebas RescueChesapeake | Form 990 | $2,880,977FY2024 |
| Norfolk Society for the Prevention of Cruelty to Animals IncNorfolk | Form 990 | $2,839,412FY2024 |
| Homeward Trails Animal Rescue IncorporatedFairfax | Form 990 | $2,645,511FY2025 |
| Society for the Prevention of Cruelty to Animals MartinsvilleMartinsville | Form 990 | $2,598,618FY2025 |
| Fauquier S P C A IncorporatedWarrenton | Form 990 | $2,510,466FY2024 |
| Front Range Equine RescueGreat Falls | Form 990 | $2,473,303FY2025 |
| Rockingham Harrisonburg Society for Prevention of Cruelty to AnimalsRockingham | Form 990 | $2,385,472FY2024 |
| Humane Society of Fairfax County IncFairfax | Form 990 | $2,368,510FY2024 |
| Peninsula Society for the Preven Tion of Cruelty to Animals IncNewport News | Form 990 | $2,260,163FY2025 |
| Lost Dog Rescue FoundationFalls Church | Form 990 | $2,109,762FY2025 |
| Project PerryLouisa | Form 990 | $2,027,523FY2024 |
| Friends of the Animal Shelter and Guardians of the Homeless AnimalsAldie | Form 990 | $1,925,855FY2025 |
| Chesapeake Humane Society IncChesapeake | Form 990 | $1,919,921FY2025 |
| Wolf Trap Animal RescueFairfax | Form 990 | $1,890,117FY2025 |
| Wounded Paw ProjectClifton | Form 990 | $1,805,379FY2024 |
| Society for the Prevention of Cruelty to Animals of FredericksbuFredericksburg | Form 990 | $1,765,474FY2025 |
| Augusta Regional SPCA IncStaunton | Form 990 | $1,751,018FY2024 |
| Heritage Humane SocietyWilliamsburg | Form 990 | $1,738,447FY2025 |
| Blue Ridge Zoological Society of Virginia IncRoanoke | Form 990 | $1,557,783FY2025 |
| Wildlife Foundation of Virginia IncNorge | Form 990 | $1,535,612FY2024 |
| Society for Integrative and Comparative BiologyHerndon | Form 990 | $1,507,605FY2025 |
| Eco Activists for Governance and Law EnforcementFairfax | Form 990 | $1,461,668FY2024 |
| Richmond Animal Welfare FoundationRichmond | Form 990 | $1,459,279FY2025 |
| Society for the Preventation of Cruelty to AnimalsOnley | Form 990 | $1,453,942FY2025 |